Enzyme Lab

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1. What will happen if an enzyme has been frozen, then it is warmed to body temperature and the substrate is added?
there is no way to predict
the reaction will occur
the reaction will not occur

2. Was the effect of very cold temperature on the enzyme reversible?


3. What type of bonds or interactions determine protein folding?
disulfate bridges
peptide bonds
”R-group” interactions
hydrogen bonds
all of these

4. Protein folding is determined by ________________ .


5. Was the effect of high temperature on the enzyme reversible?


6. Test tube “A” contains an enzyme that has been boiled and allowed to cool. If we add substrate what would you expect to see in the tube?

no foam would form
foam would form
foam would form but much less
it is impossible to predict

7. We set up another experiment in which we added the following enzymes instead of peroxidase: Test Tube A = cellulase and Test Tube B = amylase. When hydrogen peroxide is added what would you expect to see happen in the tubes?

A forms foam; B no foam
both A and B form foam
neither A nor B form foam
B forms foam; A no foam

8. Do all enzymes in the human body work best at the same temperature?


9. After we added the enzyme to a tube containing hydrogen peroxide and saw a reaction, we then tested whether there was still substrate present. Which tube tested whether we all the substrate had been used up?



10. Which of the following is FALSE? Substrates-enzyme complexes form due to
the R-groups that line the pocket
the shape of the active site
none of these are false
molecular motion causing collisions between the enzyme and the substrate
phagocytosis by the enzyme

11. Enzymes are very important to the functioning of a cell. What do enzymes do that makes them so important to life?


12. Student A, B, and C all used the same ingredients and followed directions accurately in the enzyme experiment. After mixing the ingredients they each measured a different result. Student A measured 10mm, Student B – 15mm, Student C – 12mm.
Assuming they all read the ruler accurately; give a possible explanation for the differences.

adhesion stopped some of the substrate from reaching the mixture
different size drops
different amount of flicking
all of these are possible explanations
time before measuring

13. What caused there to be little or no foam when hydrogen peroxide was added to the enzyme in the pH 2 buffer? The enzyme was ______________



14. What was the reason for returning test tubes containing pH 2 and pH 13 buffer and enzyme to the optimum pH?
to confirm the enzyme's polarity
to see if another pH was actually the optimum
to see if the enzyme was permanently denatured
to test the ability of the enzyme to catalyze the reaction
to change the amount of hydrogen ions in the solution

15. If the peroxidase in the pH 13 buffer was then returned to optimum pH, would normal foam activity develop if more H2O2 were added?


16. What aspect of the polypeptide chain makes each protein unique?


17. What is the specific reason for the folding pattern of a polypeptide chain? (at the molecular level)


18. Identify the enzyme(s) in this reaction.



19. What organ was pulverized in order to prepare the enzyme extract used in the lab?


20. Identify the substrate(s) in this reaction.